Coding

Part:BBa_K4614001:Design

Designed by: Miaodi Feng   Group: iGEM23_CAU-China   (2023-09-30)


5R5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

R5 is a short 19-amino acid peptide with multiple Lys catalytic groups that plays an important role in the formation of the siliceous cell wall of diatoms, and we wanted to use it to hydrolyze TEOS to generate silica and deposit silica on the surface of R5. 5R5 is a repeating sequence of R5 with gains in all aspects.

Source

From the genome of diatoms

References

[1]Ping H , Poudel L , Xie H , Fang W , Zou Z , Zhai P , Wagermaier W , Fratzl P , Wang W , Wang H , O'Reilly P , Ching WY , Fu Z . Synthesis of monodisperse rod-shaped silica particles through biotemplating of surface-functionalized bacteria. Nanoscale. 2020 Apr 30;12(16):8732-8741. doi: 10.1039/d0nr00669f. PMID: 32307501.